Anti-Serum Amyloid P antibody [14B4] (ab27313)

Overview

  • Product name
    Anti-Serum Amyloid P antibody [14B4]
    See all Serum Amyloid P primary antibodies
  • Description
    Mouse monoclonal [14B4] to Serum Amyloid P
  • Specificity
    None detected so far.
  • Tested applications
    Suitable for: Sandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native serum Amyloid P protein (purified) from Human plasma.

  • General notes


    This antibody reacts with serum Amyloid P coated on microtitre plates. In western blots of SDS-PAGE separated serum proteins, the antibody reacts with a high molecular weight aggregate of serum Amyloid P under non reducing conditions, and reacts strongly with a 25 kDa band of the single subunits under reducing SDS-PAGE conditions.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    This antibody reacts with serum Amyloid P coated on microtitre plates. In western blots of SDS-PAGE separated serum proteins, the antibody reacts with a high molecular weight aggregate of serum Amyloid P under non reducing conditions, and reacts strongly with a 25 kDa band of the single subunits under reducing SDS-PAGE conditions.
  • Clonality
    Monoclonal
  • Clone number
    14B4
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA 1/4000. Can be paired for Sandwich ELISA with Rabbit polyclonal to Serum Amyloid P (ab17872). Use as Capture antibody with recommended pair. See Abreview submitted by Jennifer Webster for details.
WB 1/400. Use under non reducing condition. Predicted molecular weight: 25 kDa.
ELISA 1/4000. Use at an assay dependent dilution.

Target

  • Function
    Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • Tissue specificity
    Found in serum and urine.
  • Involvement in disease
    Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • Sequence similarities
    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Post-translational
    modifications
    N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9.5S alpha 1 glycoprotein antibody
    • 9.5S alpha-1-glycoprotein antibody
    • Amyloid P component serum antibody
    • APCS antibody
    • MGC88159 antibody
    • Pentaxin related antibody
    • Pentraxin 2 antibody
    • PTX 2 antibody
    • PTX2 antibody
    • SAMP_HUMAN antibody
    • SAP antibody
    • Serum amyloid P component antibody
    • Serum amyloid P-component(1-203) antibody
    see all

References for Anti-Serum Amyloid P antibody [14B4] (ab27313)

This product has been referenced in:
  • Chen HM  et al. Amyloid P component as a plasma marker for Parkinson's disease identified by a proteomic approach. Clin Biochem 44:377-85 (2011). WB . Read more (PubMed: 21223953) »
  • Li C  et al. Pancreatic cancer serum detection using a lectin/glyco-antibody array method. J Proteome Res 8:483-92 (2009). Read more (PubMed: 19072160) »

See all 2 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Purified protein)
Loading amount
0.1 µg
Specification
Purified protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. John Andersen

Verified customer

Submitted Mar 01 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Serum (Serum)
Specification
Serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Type
Sandwich
Username

Miss. Jennifer Webster

Verified customer

Submitted Aug 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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