Overview

  • Product nameAnti-Serum Amyloid P antibody [6E6]
    See all Serum Amyloid P primary antibodies
  • Description
    Mouse monoclonal [6E6] to Serum Amyloid P
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein purified from Human plasma

  • Positive control
    • Human plasma

Properties

Applications

Our Abpromise guarantee covers the use of ab51085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 25 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • FunctionCan interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • Tissue specificityFound in serum and urine.
  • Involvement in diseaseNote=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • Sequence similaritiesBelongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Post-translational
    modifications
    N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9.5S alpha 1 glycoprotein antibody
    • 9.5S alpha-1-glycoprotein antibody
    • Amyloid P component serum antibody
    • APCS antibody
    • MGC88159 antibody
    • Pentaxin related antibody
    • Pentraxin 2 antibody
    • PTX 2 antibody
    • PTX2 antibody
    • SAMP_HUMAN antibody
    • SAP antibody
    • Serum amyloid P component antibody
    • Serum amyloid P-component(1-203) antibody
    see all

Anti-Serum Amyloid P antibody [6E6] images

  • All lanes : Anti-Serum Amyloid P antibody [6E6] (ab51085) at 1/1000 dilution

    Lane 1 : Amyloid P protein isolated from human plasma
    Lane 2 : Human plasma


    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-Serum Amyloid P antibody [6E6] (ab51085)

This product has been referenced in:
  • Gu BJ  et al. P2X7 receptor-mediated scavenger activity of mononuclear phagocytes toward non-opsonized particles and apoptotic cells is inhibited by serum glycoproteins but remains active in cerebrospinal fluid. J Biol Chem 287:17318-30 (2012). WB ; Human . Read more (PubMed: 22461619) »

See 1 Publication for this product

Product Wall

Thank you for the clarification about the blocking buffer. BSA should be fine, but if you are seeing no signal at all, you could try reducing the percentage of BSA 10-fold, to 0.5%. This might allow a signal to develop more easily.

Human ser...

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Your protocol looks mostly correct but my one concern is the antibody diluent, 5% FBS. Are you confident the Fetal Bovine Serum does not contain the target of the antibody, serum amyloid P? I think it is likely that the antibody is reacting with this i...

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