Anti-Serum Amyloid P antibody (ab17872)

Overview

  • Product name
    Anti-Serum Amyloid P antibody
    See all Serum Amyloid P primary antibodies
  • Description
    Rabbit polyclonal to Serum Amyloid P
  • Specificity
    Specific for human SAP with no crossreactivity with CRP or other pentraxins.
  • Tested applications
    Suitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Full length native protein (purified) (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab17872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/2500. Predicted molecular weight: 26.76 kDa.
ELISA 1/2500 - 1/10000.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [14B4] to Serum Amyloid P (ab27313). Use as detection antibody with recommended pair. See Abreview submitted by Jennifer Webster for details.

Target

  • Function
    Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • Tissue specificity
    Found in serum and urine.
  • Involvement in disease
    Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • Sequence similarities
    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Post-translational
    modifications
    N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9.5S alpha 1 glycoprotein antibody
    • 9.5S alpha-1-glycoprotein antibody
    • Amyloid P component serum antibody
    • APCS antibody
    • MGC88159 antibody
    • Pentaxin related antibody
    • Pentraxin 2 antibody
    • PTX 2 antibody
    • PTX2 antibody
    • SAMP_HUMAN antibody
    • SAP antibody
    • Serum amyloid P component antibody
    • Serum amyloid P-component(1-203) antibody
    see all

References

ab17872 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The reason why the customer is not seeing any bands is that normal brain has very very little SAP protein, therefore it is not surprising that the customer still sees no band. The reference below should be very useful, in particular, figure 3, whic...

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As Serum Amyloid P protein is only expressed in high levels in AD patients as is in low levels in normal subjects I think the problem is therefore low levels in the samples. Furthermore, the customer should try incubating in more concentrated antibo...

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Thank you for contacting us for technical support on behalf of your customer. Many thanks for providing so much protocol information and the images of the blot. It enabled me to understand the problem very easily and rapidly. I am a little confused...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Serum (Serum)
Specification
Serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Type
Sandwich
Username

Miss. Jennifer Webster

Verified customer

Submitted Aug 16 2006

Good morning, Unfortunately the source of ab17872 does not have an image of the western blot to provide us. I am very sorry for this inconvenience, Best of luck with your research,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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