Overview

  • Product name
    Anti-Serum Amyloid P antibody
    See all Serum Amyloid P primary antibodies
  • Description
    Rabbit polyclonal to Serum Amyloid P
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Serum Amyloid P.

  • Positive control
    • This antibody gave a positive signal in the following lysates: Human Bladder Tissue; Human Platelet Whole Cell Lysate; Human Plasma Total Protein Lysate; Human Bone Marrow Tissue; Human Spleen Tissue; Human Thymus Tissue; Human Brain Tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab66715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Function
    Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • Tissue specificity
    Found in serum and urine.
  • Involvement in disease
    Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • Sequence similarities
    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Post-translational
    modifications
    N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9.5S alpha 1 glycoprotein antibody
    • 9.5S alpha-1-glycoprotein antibody
    • Amyloid P component serum antibody
    • APCS antibody
    • MGC88159 antibody
    • Pentaxin related antibody
    • Pentraxin 2 antibody
    • PTX 2 antibody
    • PTX2 antibody
    • SAMP_HUMAN antibody
    • SAP antibody
    • Serum amyloid P component antibody
    • Serum amyloid P-component(1-203) antibody
    see all

Images

  • All lanes : Anti-Serum Amyloid P antibody (ab66715) at 1 µg/ml

    Lane 1 : Human bladder normal tissue lysate - total protein (ab29491)
    Lane 2 : Human Platelet (Human adult normal cell line) Whole Cell Lysate
    Lane 3 : Human Plasma Total Protein Lysate
    Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 5 : Human spleen tissue lysate - total protein (ab29699)
    Lane 6 : Human thymus tissue lysate - total protein (ab30146)
    Lane 7 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Anti-Serum Amyloid P antibody (ab66715) at 1 µg/ml + Natural Human Serum Amyloid P protein (ab96056) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References

ab66715 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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