This antibody gave a positive signal in the following lysates: Human Bladder Tissue; Human Platelet Whole Cell Lysate; Human Plasma Total Protein Lysate; Human Bone Marrow Tissue; Human Spleen Tissue; Human Thymus Tissue; Human Brain Tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
All lanes : Anti-Serum Amyloid P antibody (ab66715) at 1 µg/ml
Lane 1 : Human bladder normal tissue lysate - total protein (ab29491) Lane 2 : Human Platelet (Human adult normal cell line) Whole Cell Lysate Lane 3 : Human Plasma Total Protein Lysate Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue Lane 5 : Human spleen tissue lysate - total protein (ab29699) Lane 6 : Human thymus tissue lysate - total protein (ab30146) Lane 7 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa Observed band size: 25 kDa Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Western blot - Anti-Serum Amyloid P antibody (ab66715)
Anti-Serum Amyloid P antibody (ab66715) at 1 µg/ml + Natural Human Serum Amyloid P protein (ab96056) at 0.01 µg