Anti-Serum Response Factor SRF antibody (ab101246)

Overview

  • Product nameAnti-Serum Response Factor SRF antibody
    See all Serum Response Factor SRF primary antibodies
  • Description
    Rabbit polyclonal to Serum Response Factor SRF
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Serum Response Factor SRF.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HeLa Nuclear; Jurkat; HEK293; MCF7; NIH3T3; F9.

Properties

Applications

Our Abpromise guarantee covers the use of ab101246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • FunctionSRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • Sequence similaritiesContains 1 MADS-box domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

Anti-Serum Response Factor SRF antibody images

  • All lanes : Anti-Serum Response Factor SRF antibody (ab101246) at 1 µg

    Lane 1 : : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 7 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 100 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Serum Response Factor SRF antibody (ab101246)

ab101246 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab101246.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"