The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/300 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa).
Use at an assay dependent concentration.
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
Immunohistochemical analysis of Serum Response Factor SRF in paraffin embedded human breast carcinoma tissue using ab53147 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.