Overview

  • Product nameAnti-Serum Response Factor SRF antibody
    See all Serum Response Factor SRF primary antibodies
  • Description
    Rabbit polyclonal to Serum Response Factor SRF
  • Specificityab53147 detects endogenous levels of total Serum Response Factor SRF protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 99 (S-L-S-E-M).

  • Positive control
    • WB: NIH/3T3 cell extracts IHC-P: Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab53147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • FunctionSRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • Sequence similaritiesContains 1 MADS-box domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

Anti-Serum Response Factor SRF antibody images

  • All lanes : Anti-Serum Response Factor SRF antibody (ab53147) at 1/300 dilution

    Lane 1 : Extracts of NIH/3T3 cells treated with PMA (125ng/ml, 30 min) with no peptide
    Lane 2 : Extracts of NIH/3T3 cells treated with PMA (125ng/ml, 30 min) with peptide


    Predicted band size : 52 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of Serum Response Factor SRF in paraffin embedded human breast carcinoma tissue using ab53147 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.

References for Anti-Serum Response Factor SRF antibody (ab53147)

This product has been referenced in:
  • Yang C  et al. Bone marrow-derived cells may not be the original cells for carcinogen-induced mouse gastrointestinal carcinomas. PLoS One 8:e79615 (2013). IHC-Fr ; Mouse . Read more (PubMed: 24260263) »
  • Buller B  et al. Regulation of serum response factor by miRNA-200 and miRNA-9 modulates oligodendrocyte progenitor cell differentiation. Glia : (2012). Read more (PubMed: 22907787) »

See all 4 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"