Anti-Serum Response Factor SRF antibody (ab91477)

Overview

  • Product nameAnti-Serum Response Factor SRF antibody
    See all Serum Response Factor SRF primary antibodies
  • Description
    Goat polyclonal to Serum Response Factor SRF
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-QASPSRDSSTDLTQ

    , corresponding to internal sequence amino acids 368-381 of Human Serum Response Factor SRF (NP_003122.1).

  • Positive control
    • Human lung fibroblasts lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab91477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa).
Approx 67kDa band observed in primary cultured human lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (calculated MW of 51.6kDa according to NP_003122.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Davis FJ et al, Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1521-33.; PMID: 11893590).

Target

  • FunctionSRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • Sequence similaritiesContains 1 MADS-box domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

Anti-Serum Response Factor SRF antibody images

  • All lanes : Anti-Serum Response Factor SRF antibody (ab91477) at 1 µg/ml

    Lane 1 : Un-treated primary cultured human lung fibroblast lysate.
    Lane 2 : TGF-bl-treated primary cultured human lung fibroblast lysate (24hrs, 1ng/ml).

    Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References for Anti-Serum Response Factor SRF antibody (ab91477)

ab91477 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab91477.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"