The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa). Approx 67kDa band observed in primary cultured human lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (calculated MW of 51.6kDa according to NP_003122.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Davis FJ et al, Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1521-33.; PMID: 11893590).
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.