Anti-Serum Response Factor SRF antibody (ab91477)

Overview

  • Product name
    Anti-Serum Response Factor SRF antibody
    See all Serum Response Factor SRF primary antibodies
  • Description
    Goat polyclonal to Serum Response Factor SRF
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-QASPSRDSSTDLTQ

    , corresponding to internal sequence amino acids 368-381 of Human Serum Response Factor SRF (NP_003122.1).

  • Positive control
    • Human lung fibroblasts lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab91477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa).
Approx 67kDa band observed in primary cultured human lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (calculated MW of 51.6kDa according to NP_003122.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Davis FJ et al, Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1521-33.; PMID: 11893590).

Target

  • Function
    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • Sequence similarities
    Contains 1 MADS-box domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

Images

  • All lanes : Anti-Serum Response Factor SRF antibody (ab91477) at 1 µg/ml

    Lane 1 : Un-treated primary cultured human lung fibroblast lysate.
    Lane 2 : TGF-bl-treated primary cultured human lung fibroblast lysate (24hrs, 1ng/ml).

    Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References

ab91477 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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