Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130)

Overview

  • Product name
    Anti-Serum Response Factor SRF (phospho S103) antibody
    See all Serum Response Factor SRF primary antibodies
  • Description
    Rabbit polyclonal to Serum Response Factor SRF (phospho S103)
  • Specificity
    ab53130 detects endogenous levels of Serum Response Factor SRF only when phosphorylated at serine 103. In mouse and rat the modification site is serine 99.
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, EMSAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 103 (S-L-SP-E-M).

  • Positive control
    • WB: LOVO cell extracts IHC-P: Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab53130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
EMSA Use at an assay dependent concentration.

Target

  • Function
    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • Sequence similarities
    Contains 1 MADS-box domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

Images

  • All lanes : Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130) at 1/300 dilution

    Lane 1 : LOVO cell extract treated with serum (20%, 15mins)
    Lane 2 : LOVO cell extract treated with serum (20%, 15mins) with immunising peptide


    Predicted band size : 52 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

References

This product has been referenced in:
  • Heller EA  et al. Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors. Nat Neurosci 17:1720-7 (2014). Read more (PubMed: 25347353) »
  • Vissing K  et al. Effect of resistance exercise contraction mode and protein supplementation on members of the STARS signalling pathway. J Physiol 591:3749-63 (2013). Read more (PubMed: 23753523) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. Unfortunately, this specific lysate (LOVO cell extract treated with serum (20%, 15min)) is not available for sale. Alternative positive controls would be a human lung carcinoma lysate (e.g. ab14870 or ab14871...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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