Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130)
Key features and details
- Rabbit polyclonal to Serum Response Factor SRF (phospho S103)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Serum Response Factor SRF (phospho S103) antibody
See all Serum Response Factor SRF primary antibodies -
Description
Rabbit polyclonal to Serum Response Factor SRF (phospho S103) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Serum Response Factor SRF aa 50-150 (phospho S103).
Database link: P11831 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab53130 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/300 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/300 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation. -
Sequence similarities
Contains 1 MADS-box domain. -
Post-translational
modificationsPhosphorylated by PRKDC. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6722 Human
- Omim: 600589 Human
- SwissProt: P11831 Human
- Unigene: 520140 Human
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Alternative names
- c fos serum response element binding factor antibody
- c fos serum response element binding transcription factor antibody
- ELK3 antibody
see all
Images
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All lanes : Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130) at 1/300 dilution
Lane 1 : LOVO cell extract treated with serum (20%, 15mins)
Lane 2 : LOVO cell extract treated with serum (20%, 15mins) with immunising peptide
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab53130 has been referenced in 5 publications.
- Russell AP et al. Striated muscle activator of Rho signalling (STARS) is reduced in ageing human skeletal muscle and targeted by miR-628-5p. Acta Physiol (Oxf) 220:263-274 (2017). PubMed: 27739650
- Heller EA et al. Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors. Nat Neurosci 17:1720-7 (2014). PubMed: 25347353
- Vissing K et al. Effect of resistance exercise contraction mode and protein supplementation on members of the STARS signalling pathway. J Physiol 591:3749-63 (2013). PubMed: 23753523
- Kawashima T et al. Synaptic activity-responsive element in the Arc/Arg3.1 promoter essential for synapse-to-nucleus signaling in activated neurons. Proc Natl Acad Sci U S A 106:316-21 (2009). EMSA . PubMed: 19116276
- Xiao Q et al. Embryonic stem cell differentiation into smooth muscle cells is mediated by Nox4-produced H2O2. Am J Physiol Cell Physiol 296:C711-23 (2009). PubMed: 19036941