Anti-Seryl-tRNA synthetase antibody [EPR10684(B)] (ab154825)


  • Product name
    Anti-Seryl-tRNA synthetase antibody [EPR10684(B)]
    See all Seryl-tRNA synthetase primary antibodies
  • Description
    Rabbit monoclonal [EPR10684(B)] to Seryl-tRNA synthetase
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminal of Human Seryl-tRNA synthetase.

  • Positive control
    • 293T and K562 cell lysates; Human pancreas tissue and Human thyroid gland carcinoma tissue; 293T cells and K562 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab154825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.



  • Function
    Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
  • Pathway
    Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
  • Sequence similarities
    Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.
  • Domain
    Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • C78314 antibody
    • cytoplasmic antibody
    • EC antibody
    • FLJ36399 antibody
    • sarS antibody
    • Sars1 antibody
    • Serine tRNA ligase 1, cytoplasmic antibody
    • Serine tRNA ligase antibody
    • Serine--tRNA ligase antibody
    • serine--tRNA ligase, cytoplasmic antibody
    • SerRS antibody
    • SERS antibody
    • Seryl tRNA Ser/Sec synthetase antibody
    • Seryl tRNA synthetase antibody
    • Seryl tRNA synthetase cytoplasmic antibody
    • Seryl-tRNA Ser/Sec synthetase antibody
    • Seryl-tRNA synthetase antibody
    • seryl-tRNA synthetase, cytoplasmic antibody
    • Seryl-tRNA(Ser/Sec) synthetase antibody
    • Strs antibody
    • SYSC_HUMAN antibody
    see all


  • All lanes : Anti-Seryl-tRNA synthetase antibody [EPR10684(B)] (ab154825) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 59 kDa

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Seryl-tRNA synthetase with ab154825 at 1/50 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Seryl-tRNA synthetase with ab154825 at 1/50 dilution.
  • Immunofluorescence analysis of 293T cells labeling Seryl-tRNA synthetase with ab154825 at 1/100 dilution.
  • Flow cytometric analysis of permeabilized K562 cells labeling Seryl-tRNA synthetase with ab154825 at 1/100 dilution (red) or a negative Rabbit IgG (green).


This product has been referenced in:
  • Xu L  et al. Three distinct 3-methylcytidine (m3C) methyltransferases modify tRNA and mRNA in mice and humans. J Biol Chem 292:14695-14703 (2017). Read more (PubMed: 28655767) »

See 1 Publication for this product

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