Overview

  • Product nameAnti-SESN1 antibody
    See all SESN1 primary antibodies
  • Description
    Mouse polyclonal to SESN1
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-551 of Human SESN1

  • Positive control
    • HeLa cells (IF/ICC) Transfected 293T cell line (WB)

Properties

Applications

Our Abpromise guarantee covers the use of ab67156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use a concentration of 10 µg/ml. Requires purification with Protein A prior to ICC/IF.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionInvolved in the reduction of peroxiredoxins. May also be regulator of cellular growth.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the sestrin family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110002G11Rik antibody
    • AU044290 antibody
    • MGC118148 antibody
    • MGC138241 antibody
    • MGC142129 antibody
    • OTTMUSP00000022799 antibody
    • p53 activated gene 26 antibody
    • p53 regulated PA26 nuclear protein antibody
    • p53 regulated protein PA26 antibody
    • p53-regulated protein PA26 antibody
    • PA26 antibody
    • RP11-787I22.1 antibody
    • sesn1 antibody
    • SESN1_HUMAN antibody
    • SEST1 antibody
    • sestrin 1 antibody
    • Sestrin-1 antibody
    see all

Anti-SESN1 antibody images

  • All lanes : Anti-SESN1 antibody (ab67156) at 1/500 dilution

    Lane 1 : transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

    Predicted band size : 57 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of ab67156 at 10 µg/ml on HeLa cells
  • IHC image of ab67156 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67156, at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-SESN1 antibody (ab67156)

ab67156 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"