Overview

  • Product nameAnti-SESN3 antibody
    See all SESN3 primary antibodies
  • Description
    Rabbit polyclonal to SESN3
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 21 and 266 of Human SESN3 (NM_144665).

  • Positive control
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: A549 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
ICC/IF 1/100 - 1/200.

Target

Anti-SESN3 antibody images

  • Anti-SESN3 antibody (ab97792) at 1/1000 dilution + Hela whole cell lysate at 30 µg

    Predicted band size : 57 kDa
  • Immunofluorescence analysis of SESN3 in paraformaldehyde fixed A549, using ab97792 at a 1/200 dilution. The lower image was merged with DNA probe.

References for Anti-SESN3 antibody (ab97792)

This product has been referenced in:
  • Park HW  et al. Hepatoprotective role of Sestrin2 against chronic ER stress. Nat Commun 5:4233 (2014). Read more (PubMed: 24947615) »
  • Kang X  et al. The inhibitory effect of ethanol on Sestrin3 in the pathogenesis of ethanol-induced liver injury. Am J Physiol Gastrointest Liver Physiol 307:G58-65 (2014). Read more (PubMed: 24833709) »

See all 2 Publications for this product

Product Wall

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 100 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (8)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"