Overview

  • Product nameAnti-SET antibody
    See all SET primary antibodies
  • Description
    Rabbit polyclonal to SET
  • SpecificityReactive with I2aPP2A Does not react with I2bPP2A.
  • Tested applicationsSuitable for: IHC-P, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 3-18 of Human I2 alpha PP2A.

Properties

Applications

Our Abpromise guarantee covers the use of ab1183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionMultitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
  • Tissue specificityWidely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
  • Involvement in diseaseNote=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
  • Sequence similaritiesBelongs to the nucleosome assembly protein (NAP) family.
  • DomainThe C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.
  • Post-translational
    modifications
    Isoform 2 is phosphorylated on Ser-15 and Thr-23.
    Isoform 2 is acetylated on Lys-11.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
    N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.
  • Cellular localizationCytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2PP2A antibody
    • HLA DR associated protein II antibody
    • HLA-DR-associated protein II antibody
    • I 2PP2A antibody
    • I-2PP2A antibody
    • I2PP2A antibody
    • IGAAD antibody
    • Inhibitor of granzyme A activated DNase antibody
    • Inhibitor of granzyme A-activated DNase antibody
    • Inhibitor of GzmA-activated DNase antibody
    • inhibitor-2 of protein phosphatase-2A antibody
    • IPP2A2 antibody
    • PHAPII antibody
    • Phosphatase 2A inhibitor I2PP2A antibody
    • protein phosphatase type 2A inhibitor antibody
    • Protein SET antibody
    • Set antibody
    • SET nuclear oncogene antibody
    • SET translocation antibody
    • SET translocation (myeloid leukemia-associated) antibody
    • SET_HUMAN antibody
    • TAF I antibody
    • TAF IBETA antibody
    • TAF-I antibody
    • TAFI antibody
    • Template activating factor I antibody
    • Template-activating factor I antibody
    • Template-Activating Factor-I, chromatin remodelling factor antibody
    see all

Anti-SET antibody images

  • Anti-SET antibody (ab1183) at 1/2000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Anti-rabbit IgG HRP-conjugate polyclonal at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Western blot of 10 µg HEK 293 cell extracts with Anti- I2α (GLO150). The band corresponds to I2αPP2A (~ 39 kDa).

    Western blot of 10 µg HEK 293 cell extracts with Anti- I2a (GLO150). The band corresponds to I2aPP2A (~ 39 kDa).
  • ICC/IF image of ab1183 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1183, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab35252 (4µg/ml) staining SET in human kidney cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-SET antibody (ab1183)

This product has been referenced in:
  • Yabe R  et al. Characterization of SET/I2PP2A isoforms in dogs. J Vet Med Sci 76:1235-40 (2014). WB . Read more (PubMed: 24897959) »
  • Mansouri S  et al. A role for the nucleosome assembly proteins TAF-Iß and NAP1 in the activation of BZLF1 expression and Epstein-Barr virus reactivation. PLoS One 8:e63802 (2013). WB ; Human . Read more (PubMed: 23691099) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (EC cells)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 60 µg
Specification EC cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Apr 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12 Bis tris)
Sample Human Cell lysate - nuclear (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Dr. Paul Lesbats

Verified customer

Submitted Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 11 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Sample Human Cell lysate - whole cell (HeLa cell lysate)
Specification HeLa cell lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 31 2013

Thank you for contacting us.

The immunogen sequence corresponds to residues 3 to 18 of the second isoform listed below, the first one that was discovered and sequenced.

transcript variant 2: http://www.ncbi.nlm.nih.gov/nuccore/NM_0...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse oocytes)
Specification mouse oocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100 in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Username

Dr. qi shutao

Verified customer

Submitted Jul 14 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse oocytes)
Loading amount 200 cells
Specification mouse oocytes
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Dr. qi shutao

Verified customer

Submitted Jul 14 2010

Thank you for your email and I apologize for the shortage of ab1183 that you received. A free of charge replacement vial is being sent to your attention on order reference # 111400. The antibody is out of stock at the moment and the expected delivery d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"