Anti-SETBP1 antibody (ab68715)

Overview

  • Product name
  • Description
    Mouse polyclonal to SETBP1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein (Human) SETBP1 (AAH62338.1, 242 a.a)

  • Positive control
    • SETBP1 transfected 293T lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).

Target

  • Tissue specificity
    Expressed in numerous tissues.
  • Involvement in disease
    Defects in SETBP1 are the cause of Schinzel-Giedion midface retraction syndrome (SGMFS) [MIM:269150]. It is a disorder characterized by severe mental retardation, distinctive facial features, and multiple congenital malformations including skeletal abnormalities, genitourinary and renal malformations, cardiac defects, as well as a higher-than-normal prevalence of tumors, notably neuroepithelial neoplasia.
  • Sequence similarities
    Contains 3 A.T hook DNA-binding domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • SEB antibody
    • SET binding protein 1 antibody
    • SET-binding protein antibody
    • SETBP 1 antibody
    • SETBP_HUMAN antibody
    • Setbp1 antibody
    see all

Images

  • All lanes : Anti-SETBP1 antibody (ab68715) at 1/500 dilution

    Lane 1 : SETBP1 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 27 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

References

ab68715 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) R...

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Thank you for your email.

I would be happy to process refunds for ab213 and ab68715. Could you however let me know why the customer expecting a 170kDa band for ab58101 which should be nearly 130-140 kDa.

Thanks!

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

-...

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You are correct that ab68715 and ab98222 are both directed against SETBP1. The western blot data show detection of different molecular weight bands becuase the samples run on the blots are different. In the blot for ab98222, the sample is fetal st...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Serum (serum (with pancreatict tumor development))
Loading amount
50 µg
Specification
serum (with pancreatict tumor development)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 23 2012

Thank you for your inquiry.

Unfortunately, I am unable to issue discount code for the following antibodies:

ab60720, ab86704 and ab65109.

The immunogens for the MUC antibodies do not show a high enough homology (70% and be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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