Anti-SETD7 antibody [2D10] (ab119400)

Overview

  • Product nameAnti-SETD7 antibody [2D10]
    See all SETD7 primary antibodies
  • Description
    Mouse monoclonal [2D10] to SETD7
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length Human SETD7 produced in HEK293T cells (NP_085151).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY SETD7 cDNA; HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cell extracts; Human endometrium adenocarcinoma and bladder carcinoma tissues; COS7 cells transiently transfected by pCMV6-ENTRY SETD7; HEK293T cells transfected with pCMV6-ENTRY SETD7 overexpress plasmid.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab119400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 41 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionHistone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
  • Tissue specificityWidely expressed. Expressed in pancreatic islets.
  • Sequence similaritiesBelongs to the histone-lysine methyltransferase family. SET7 subfamily.
    Contains 3 MORN repeats.
    Contains 1 SET domain.
  • DomainThe SET domain is necessary but not sufficient for histone methyltransferase activity.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ21193 antibody
    • H3 K4 HMTase antibody
    • H3-K4-HMTase SETD7 antibody
    • H4 lysine 4 specific antibody
    • Histone H3 K4 methyltransferase antibody
    • Histone H3 lysine 4 specific methyltransferase antibody
    • Histone H3-K4 methyltransferase SETD7 antibody
    • Histone H4 K4 methyltransferase antibody
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody
    • Histone-lysine N-methyltransferase SETD7 antibody
    • KIAA1717 antibody
    • KMT7 antibody
    • Lysine methyltransferase antibody
    • Lysine N-methyltransferase 7 antibody
    • OTTHUMP00000164543 antibody
    • OTTHUMP00000220049 antibody
    • SET 7 antibody
    • SET 7/9 antibody
    • SET 9 antibody
    • SET D7 antibody
    • SET domain containing (lysine methyltransferase) 7 antibody
    • SET domain containing protein 7 antibody
    • SET domain containing protein 8 antibody
    • SET domain-containing protein 7 antibody
    • SET7 antibody
    • SET7/9 antibody
    • SET9 antibody
    • Setd7 antibody
    • SETD7_HUMAN antibody
    see all

Anti-SETD7 antibody [2D10] images

  • All lanes : Anti-SETD7 antibody [2D10] (ab119400) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SETD7 cDNA

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique

    Predicted band size : 41 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC219244)
  • All lanes : Anti-SETD7 antibody [2D10] (ab119400) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 41 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC219244)
  • ab111155 at 1/150 dilution staining SETD7 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab111155 at 1/150 dilution staining SETD7 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab119400 at 1/100 dilution staining SETD7 in COS7 cells transiently transfected with pCMV6-ENTRY SETD7, by Immunofluorescence
  • ab119400 at 1/100 dilution staining SETD7 in HEK293T cells transfected with either pCMV6-ENTRY SETD7 overexpress plasmid (Red) or empty vector control plasmid (Blue) and then analysed by Flow Cytometry.

References for Anti-SETD7 antibody [2D10] (ab119400)

ab119400 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Aug 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"