Overview

  • Product name
    Anti-SETD7 antibody - C-terminal
    See all SETD7 primary antibodies
  • Description
    Rabbit polyclonal to SETD7 - C-terminal
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the C terminal region of human KMT7/ SETD7/ SET7/9 conjugated to KLH.

  • Positive control
    • Mouse brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).

Target

  • Function
    Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
  • Tissue specificity
    Widely expressed. Expressed in pancreatic islets.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
    Contains 3 MORN repeats.
    Contains 1 SET domain.
  • Domain
    The SET domain is necessary but not sufficient for histone methyltransferase activity.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ21193 antibody
    • H3 K4 HMTase antibody
    • H3-K4-HMTase SETD7 antibody
    • H4 lysine 4 specific antibody
    • Histone H3 K4 methyltransferase antibody
    • Histone H3 lysine 4 specific methyltransferase antibody
    • Histone H3-K4 methyltransferase SETD7 antibody
    • Histone H4 K4 methyltransferase antibody
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody
    • Histone-lysine N-methyltransferase SETD7 antibody
    • KIAA1717 antibody
    • KMT7 antibody
    • Lysine methyltransferase antibody
    • Lysine N-methyltransferase 7 antibody
    • OTTHUMP00000164543 antibody
    • OTTHUMP00000220049 antibody
    • SET 7 antibody
    • SET 7/9 antibody
    • SET 9 antibody
    • SET D7 antibody
    • SET domain containing (lysine methyltransferase) 7 antibody
    • SET domain containing protein 7 antibody
    • SET domain containing protein 8 antibody
    • SET domain-containing protein 7 antibody
    • SET7 antibody
    • SET7/9 antibody
    • SET9 antibody
    • Setd7 antibody
    • SETD7_HUMAN antibody
    see all

Images

  • Anti-SETD7 antibody - C-terminal (ab71214) at 1/70 dilution + Mouse brain tissue lysate at 35 µg

    Predicted band size : 41 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Chen L  et al. Transcriptional control in cardiac progenitors: Tbx1 interacts with the BAF chromatin remodeling complex and regulates Wnt5a. PLoS Genet 8:e1002571 (2012). WB ; Mouse . Read more (PubMed: 22438823) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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