The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesChIP: Use at an assay dependent dilution (PMID 17578910).
WB: 1/500 - 1/1000. Detects a band of approximately 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHistone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
Tissue specificityWidely expressed. Expressed in pancreatic islets.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. SET7 subfamily. Contains 3 MORN repeats. Contains 1 SET domain.
DomainThe SET domain is necessary but not sufficient for histone methyltransferase activity.
Histone H3 lysine 4 specific methyltransferase antibody
Histone H3-K4 methyltransferase SETD7 antibody
Histone H4 K4 methyltransferase antibody
Histone lysine N methyltransferase antibody
Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody
Histone-lysine N-methyltransferase SETD7 antibody
Lysine methyltransferase antibody
Lysine N-methyltransferase 7 antibody
SET 7 antibody
SET 7/9 antibody
SET 9 antibody
SET D7 antibody
SET domain containing (lysine methyltransferase) 7 antibody
SET domain containing protein 7 antibody
SET domain containing protein 8 antibody
SET domain-containing protein 7 antibody
Anti-SETD7 antibody - ChIP Grade images
Western blot - Anti-SETD7 antibody - ChIP Grade (ab13731)
Western blot analysis fof Set7 using ab13731 at 1:500 on 20 ugs of HeLa whole cell lysate.
References for Anti-SETD7 antibody - ChIP Grade (ab13731)
This product has been referenced in:
Narayanan A et al. The coactivator host cell factor-1 mediates Set1 and MLL1 H3K4 trimethylation at herpesvirus immediate early promoters for initiation of infection. Proc Natl Acad Sci U S A104:10835-40 (2007).
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