Anti-Metnase antibody (ab129455)
Key features and details
- Rabbit polyclonal to Metnase
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Metnase antibody
See all Metnase primary antibodies -
Description
Rabbit polyclonal to Metnase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Metnase aa 1-50 (N terminal). (BAD96454.1)
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Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab129455 is purified using an epitope specific to Metnase immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 77 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 77 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 'Lys-36'. Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA. -
Tissue specificity
Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle. -
Sequence similarities
In the N-terminal section; belongs to the histone-lysine methyltransferase family.
In the C-terminal section; belongs to the mariner transposase family.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
Domain
The mariner transposase Hsmar1 region mediates DNA-binding. It has no transposase activity because the active site contains an Asn in position 610 instead of a Asp residue. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 6419 Human
- Omim: 609834 Human
- SwissProt: Q53H47 Human
- Unigene: 716054 Human
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Alternative names
- Histone lysine N methyltransferase antibody
- Histone lysine N methyltransferase SETMAR antibody
- Hsmar 1 antibody
see all
Images
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All lanes : Anti-Metnase antibody (ab129455) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 30 seconds -
Detection of Metnase by Western Blot of Immunoprecipitates.
ab129455 at 1µg/ml staining Metnase in HeLa whole cell lysate immunoprecipitated using ab129455 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab129455 has been referenced in 4 publications.
- Lié O et al. SETMAR Shorter Isoform: A New Prognostic Factor in Glioblastoma. Front Oncol 11:638397 (2021). PubMed: 35047379
- Sharma N et al. Distinct roles of structure-specific endonucleases EEPD1 and Metnase in replication stress responses. NAR Cancer 2:zcaa008 (2020). PubMed: 32743552
- Dussaussois-Montagne A et al. SETMAR isoforms in glioblastoma: A matter of protein stability. Oncotarget 8:9835-9848 (2017). PubMed: 28038463
- Carlson SM et al. A Proteomic Strategy Identifies Lysine Methylation of Splicing Factor snRNP70 by the SETMAR Enzyme. J Biol Chem 290:12040-7 (2015). PubMed: 25795785