Overview

  • Product name
  • Description
    Goat polyclonal to SETMAR
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    RWQKCVDCNGSYFD

    , corresponding to C terminal amino acids 658-671 of Human SETMAR.

  • Positive control
    • Daudi lysate.
  • General notes
    Principal Names – SETMAR; SET domain and mariner transposase fusion gene. Official Gene Symbol - SETMAR. GenBank Accession Number – NP_006506. LocusLink ID - 6419 (human). Gene Ontology terms - transposase activity

Properties

Applications

Our Abpromise guarantee covers the use of ab3823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70-75 kDa.Can be blocked with Human SETMAR peptide (ab22960).

Approx 70 - 75 kDa band seen in Daudi lysate.

Target

  • Function
    Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 'Lys-36'. Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA.
  • Tissue specificity
    Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle.
  • Sequence similarities
    In the N-terminal section; belongs to the histone-lysine methyltransferase family.
    In the C-terminal section; belongs to the mariner transposase family.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • Domain
    The mariner transposase Hsmar1 region mediates DNA-binding. It has no transposase activity because the active site contains an Asn in position 610 instead of a Asp residue.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase SETMAR antibody
    • Hsmar 1 antibody
    • Hsmar1 antibody
    • Mariner transposase Hsmar1 antibody
    • Metnase antibody
    • SET domain and mariner transposase fusion antibody
    • SET domain and mariner transposase fusion gene antibody
    • SET domain and mariner transposase fusion gene containing protein antibody
    • SET domain and mariner transposase fusion gene-containing protein antibody
    • Setmar antibody
    • SETMR_HUMAN antibody
    see all

Images

  • ab3823 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hours.  Detected by western blot using chemiluminescence. ab3823 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.

References

This product has been referenced in:
  • Van Duyne R  et al. Lysine methylation of HIV-1 Tat regulates transcriptional activity of the viral LTR. Retrovirology 5:40 (2008). WB ; Human . Read more (PubMed: 18498648) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you very much for your patience. The only suggestion that I have at this point (you have done pretty much everything!) is to try blocking with 3% BSA instead. The originator didn't see high background with this antibody and used a more sensitive ...

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The samples (in 1.5ml microtubes) were simply put into boiling water. So I would expect the temperature to be almost exactly 100 degrees centigrade.

As stated on the datasheet we used the following conditions to get our result: ab3823 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence ...

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