The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
Isoform 1 and isoform 2 are present in liver and testis.
Contains 2 laminin G-like domains.
Secreted. In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.
ab110140, at 3.75 µg/ml, staining Sex Hormone Binding Globulin in Human placenta tissue by immunohistochemistry. After incubation with ab110140, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.