This antibody gave a positive signal in HepG2 as well as the following Human tissue lysates: Liver; Fetal Liver; Testis.
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 43 kDa).
FunctionFunctions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
Tissue specificityIsoform 1 and isoform 2 are present in liver and testis.
ab119436 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab119436 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-Sex Hormone Binding Globulin antibody (ab119436)
All lanes : Anti-Sex Hormone Binding Globulin antibody (ab119436) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889) Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282) Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Human testis tissue lysate - total protein (ab30257) Lane 5 : Human liver tissue lysate - total protein (ab29889) with Immunizing peptide at 1 µg/ml Lane 6 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282) with Immunizing peptide at 1 µg/ml Lane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml Lane 8 : Human testis tissue lysate - total protein (ab30257) with Immunizing peptide at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Exposure time : 4 minutesThe band observed at 37 kDa could potentially be a cleaved form of Sex Hormone Binding Globulin due to the presence of a 29 amino acid signal peptide.
References for Anti-Sex Hormone Binding Globulin antibody (ab119436)
This product has been referenced in:
Zhao C et al. Early second-trimester plasma protein profiling using multiplexed isobaric tandem mass tag (TMT) labeling predicts gestational diabetes mellitus. Acta Diabetol52:1103-12 (2015).
Read more (PubMed: 26259496) »