Customer reviews and FAQs

Overview

  • Product nameAnti-Sez6 antibody
    See all Sez6 primary antibodies
  • Description
    Rabbit polyclonal to Sez6
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to amino acids 774-897 of Human Sez6 (UniProt: Q53EL9).

  • Positive control
    • Human hippocampus tissue and plasma.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay play a role in cell-cell recognition and in neuronal membrane signaling. Seems to be important for the achievement of the necessary balance between dendrite elongation and branching during the elaboration of a complex dendritic arbor. Involved in the development of appropriate excitatory synaptic connectivity.
  • Sequence similaritiesBelongs to the SEZ6 family.
    Contains 2 CUB domains.
    Contains 5 Sushi (CCP/SCR) domains.
  • Post-translational
    modifications    Glycosylated.
  • Cellular localizationCell membrane. Localized on dendrites and in the synaptic and postsynaptic fraction.
  • Information by UniProt
  • Database links
    • Alternative names
      • Brain-specific receptor-like protein C antibody
      • BSRP-C antibody
      • hSEZ-6 antibody
      • Seizure protein 6 antibody
      • Seizure protein 6 homolog antibody
      • Seizure related gene 6 antibody
      • SEZ-6 antibody
      • Sez6 antibody
      • SEZ6_HUMAN antibody
      see all

    Anti-Sez6 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody (ab121347)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody (ab121347)This image is courtesy of an Abreview submitted by Hyun Jin Kim

      ab121347 staining Sez6 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 4% serum for 1 hour at 4°C; antigen retrieval used 0.1% NaBh4. Samples were incubated with primary antibody (undiluted) for 24 hours at 4°C. An undiluted Cy3®-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody (ab121347)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody (ab121347)
      ab121347, at 1/25, staining Sez6 in paraffin-embedded Human Hippocampus tissue by Immunohistochemistry.

    References for Anti-Sez6 antibody (ab121347)

    ab121347 has not yet been referenced specifically in any publications.

    Publishing research using ab121347? Please let us know so that we can cite the reference in this datasheet.

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    Submit an abreview for ab121347 Submit a question for ab121347

    1 Abreview or Q&A

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    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Sez6 antibody

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
    Antigen retrieval step Other
    Sample Mouse Tissue sections (Fixed mouse brain)
    Specification Fixed mouse brain
    Permeabilization Yes - 0.5% TritonX 100
    Fixative Paraformaldehyde
    Read More
    abreview image
    Username

    Dr. Hyun Jin Kim

    Verified customer

    Submitted Aug 09 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"