Overview

  • Product nameAnti-Sez6 antibody
    See all Sez6 primary antibodies
  • Description
    Rabbit polyclonal to Sez6
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to amino acids 774-897 of Human Sez6 (UniProt: Q53EL9).

  • Positive control
    • Human hippocampus tissue and plasma.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay play a role in cell-cell recognition and in neuronal membrane signaling. Seems to be important for the achievement of the necessary balance between dendrite elongation and branching during the elaboration of a complex dendritic arbor. Involved in the development of appropriate excitatory synaptic connectivity.
  • Sequence similaritiesBelongs to the SEZ6 family.
    Contains 2 CUB domains.
    Contains 5 Sushi (CCP/SCR) domains.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationCell membrane. Localized on dendrites and in the synaptic and postsynaptic fraction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain-specific receptor-like protein C antibody
    • BSRP-C antibody
    • hSEZ-6 antibody
    • Seizure protein 6 antibody
    • Seizure protein 6 homolog antibody
    • Seizure related gene 6 antibody
    • SEZ-6 antibody
    • Sez6 antibody
    • SEZ6_HUMAN antibody
    see all

Anti-Sez6 antibody images

  • ab121347 staining Sez6 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 4% serum for 1 hour at 4°C; antigen retrieval used 0.1% NaBh4. Samples were incubated with primary antibody (undiluted) for 24 hours at 4°C. An undiluted Cy3®-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab121347, at 1/25, staining Sez6 in paraffin-embedded Human Hippocampus tissue by Immunohistochemistry.

References for Anti-Sez6 antibody (ab121347)

ab121347 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Antigen retrieval step Other
Sample Mouse Tissue sections (Fixed mouse brain)
Specification Fixed mouse brain
Permeabilization Yes - 0.5% TritonX 100
Fixative Paraformaldehyde
Username

Dr. Hyun Jin Kim

Verified customer

Submitted Aug 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"