Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SF2.
(Peptide available as ab38811.)
Our Abpromise guarantee covers the use of ab38017 in the following tested applications.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
This image is courtesy of an anonymous Abreview
IHC image of SF2 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.