Overview

  • Product name
  • Description
    Rabbit polyclonal to SF3A1
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: QQTTQQQLPQ KVQAQVIQET IVPKEPPPEF EFIADPPSIS AFDLDVVKLT, corresponding to amino acids 121-170 of Human SF3A1

  • Positive control
    • 721 B lymphoblast cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • Function
    Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Contains 2 SURP motif repeats.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Pre mRNA processing 21 antibody
    • Pre mRNA splicing factor SF3a antibody
    • PRP21 antibody
    • PRPF21 antibody
    • SAP 114 antibody
    • SAP114 antibody
    • sf3a1 antibody
    • SF3A1_HUMAN antibody
    • SF3a120 antibody
    • Spliceosome associated protein 114 antibody
    • Spliceosome-associated protein 114 antibody
    • Splicing factor 3 subunit 1 antibody
    • Splicing factor 3a subunit 1 120kDa antibody
    • Splicing factor 3A subunit 1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SF3A1 with ab69903 at 1/250. Cells wew fixed with 4% PFA for 30 minutes and blocked with 5% goat serum + 1% BSA + 0.02% fish gelatin overnight at 4°C. Cells were incubated with the primary antibody overnight at 4°C. An Alexa Fluor 546-conjugated anti-rabbit IgG (1/500, room temperature, 2 hours) was used as the secondary antibody.

  • Anti-SF3A1 antibody (ab69903) at 0.25 µg/ml + 721 B lymphoblast cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)

References

ab69903 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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