• Product nameAnti-SF3A1 antibody
    See all SF3A1 primary antibodies
  • Description
    Rabbit polyclonal to SF3A1
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: QQTTQQQLPQ KVQAQVIQET IVPKEPPPEF EFIADPPSIS AFDLDVVKLT, corresponding to amino acids 121-170 of Human SF3A1

  • Positive control
    • 721 B lymphoblast cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab69903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.


  • FunctionSubunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesContains 2 SURP motif repeats.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Pre mRNA processing 21 antibody
    • Pre mRNA splicing factor SF3a antibody
    • PRP21 antibody
    • PRPF21 antibody
    • SAP 114 antibody
    • SAP114 antibody
    • sf3a1 antibody
    • SF3A1_HUMAN antibody
    • SF3a120 antibody
    • Spliceosome associated protein 114 antibody
    • Spliceosome-associated protein 114 antibody
    • Splicing factor 3 subunit 1 antibody
    • Splicing factor 3a subunit 1 120kDa antibody
    • Splicing factor 3A subunit 1 antibody
    see all

Anti-SF3A1 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SF3A1 with ab69903 at 1/250. Cells wew fixed with 4% PFA for 30 minutes and blocked with 5% goat serum + 1% BSA + 0.02% fish gelatin overnight at 4°C. Cells were incubated with the primary antibody overnight at 4°C. An Alexa Fluor 546-conjugated anti-rabbit IgG (1/500, room temperature, 2 hours) was used as the secondary antibody.

  • Anti-SF3A1 antibody (ab69903) at 0.25 µg/ml + 721 B lymphoblast cell lysate at 10 µg

    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)

References for Anti-SF3A1 antibody (ab69903)

ab69903 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab69903.
Please use the links above to contact us or submit feedback about this product.