Overview

  • Product name
  • Description
    Rabbit polyclonal to SF3A1
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 300 and 350 of Human SF3A1 (NP_005868.1).

  • Positive control
    • Human Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Small Cell Lung Cancer, Stomach Adenocarcinoma and Testicular Seminoma. Mouse Renal Cell Carcinoma and Teratoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Contains 2 SURP motif repeats.
      Contains 1 ubiquitin-like domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Pre mRNA processing 21 antibody
      • Pre mRNA splicing factor SF3a antibody
      • PRP21 antibody
      • PRPF21 antibody
      • SAP 114 antibody
      • SAP114 antibody
      • sf3a1 antibody
      • SF3A1_HUMAN antibody
      • SF3a120 antibody
      • Spliceosome associated protein 114 antibody
      • Spliceosome-associated protein 114 antibody
      • Splicing factor 3 subunit 1 antibody
      • Splicing factor 3a subunit 1 120kDa antibody
      • Splicing factor 3A subunit 1 antibody
      see all

    Images

    • ab84698, at 1/250 dilution, staining SF3A1 in formalin-fixed, paraffin-embedded human pancreatic islet cell tumor by Immunohistochemistry, using DAB staining.
    • ab84698, at 1/100 dilution, staining SF3A1 in formalin-fixed, paraffin-embedded mouse teratoma by Immunohistochemistry, using DAB staining.
    • ab84698, at 1/100 dilution, staining SF3A1 in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using a Red fluorescent goat anti-rabbit IgG highly cross adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.

    References

    ab84698 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab84698.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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