Overview

  • Product nameAnti-SF3A3 antibody
    See all SF3A3 primary antibodies
  • Description
    Mouse monoclonal to SF3A3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-502 of Human SF3A3

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Ascites fluid.
  • Concentration information loading...
  • PurityAscites
  • ClonalityMonoclonal
  • IsotypeIgM
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.

Target

  • RelevanceSF3A3 is subunit 3 of the splicing factor SF3A. Splicing factor 3a is a heterotrimer consisting of subunits 1, 2 and 3, and is necessary for the conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. SF3A interacts with the pre-mRNA branch site early during spliceosome formation.
  • Cellular localizationNucleus speckle.
  • Database links
  • Alternative names
    • SF3A3 antibody
    • Pre mRNA splicing factor SF3a (60kD) antibody
    • Pre mRNA splicing factor SF3a antibody
    • PRP 9 antibody
    • PRP9 antibody
    • PRPF 9 antibody
    • PRPF9 antibody
    • SAP 61 antibody
    • SAP61 antibody
    • SF3a60 antibody
    • Spliceosome associated protein 61 antibody
    • Splicing factor 3a subunit 3 60kDa antibody
    • Splicing factor 3a subunit 3 antibody
    see all

Anti-SF3A3 antibody images



  • Predicted band size : 59 kDa
    SF3A3 antibody (ab56823) at 1ug/lane + A-431 cell lysate at 25ug/lane.

References for Anti-SF3A3 antibody (ab56823)

This product has been referenced in:
  • Yoshimoto R  et al. Isolation and characterization of post-splicing lariat-intron complexes. Nucleic Acids Res : (2008). WB ; Human . Read more (PubMed: 19103666) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Fibroblast)
Loading amount 10 µg
Specification Human Fibroblast
Gel Running Conditions Reduced Denaturing (4-12% Nupage)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"