Anti-SF3B14 antibody (ab74676)
Key features and details
- Rabbit polyclonal to SF3B14
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SF3B14 antibody -
Description
Rabbit polyclonal to SF3B14 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthesized peptide derived from the C-terminal region of human SF3B14
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 14 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 14 kDa. |
Target
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Function
Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51639 Human
- Entrez Gene: 66055 Mouse
- Omim: 607835 Human
- SwissProt: Q9Y3B4 Human
- SwissProt: P59708 Mouse
- Unigene: 177861 Human
- Unigene: 102627 Mouse
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Alternative names
- Ht006 antibody
- P14 antibody
- PM14_HUMAN antibody
see all
Images
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All lanes : Anti-SF3B14 antibody (ab74676) at 1/500 dilution
Lane 1 : extracts from HepG2 cells
Lane 2 : extracts from HepG2 cells with immunizing peptide at 10 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 14 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab74676 has not yet been referenced specifically in any publications.