The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 98 kDa.
Use a concentration of 6 µg/ml.
Use a concentration of 60 µg/ml.
RelevanceSF3B2 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B2 may also be involved in the assembly of the 'E' complex. It also belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
ab56800, at 60 µg/ml staining SF3B2 in HeLa Cells by immunocytochemistry.Immunofluorescence of ab56800 to SF3B2 on HeLa cell, performed with antibody concentration at 60 ug/ml.
References for Anti-SF3B2 antibody (ab56800)
This product has been referenced in:
Lin-Moshier Y et al. Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling. J Biol Chem288:355-67 (2013).
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