Overview

  • Product nameAnti-SF3B2 antibody
    See all SF3B2 primary antibodies
  • Description
    Mouse monoclonal to SF3B2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length Human SF3B2 protein (AAH07610, 1 a.a. ~ 637 a.a) with a tag of 26 kDa.

  • Positive control
    • HeLa nuclear lysate. Recombinant tagged full length human SF3B2 protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab77154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 98 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceSF3B2 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B2 may also be involved in the assembly of the 'E' complex. It also belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Pre mRNA splicing factor SF3b 145 kDa subunit antibody
    • SAP 145 antibody
    • SAP145 antibody
    • SF3b1 antibody
    • SF3B145 antibody
    • SF3b150 antibody
    • Spliceosome associated protein 145 antibody
    • Splicing factor 3b subunit 2 145kDa antibody
    • Splicing factor 3b subunit 2 antibody
    see all

Anti-SF3B2 antibody images

  • Anti-SF3B2 antibody (ab77154) at 1 µg/ml + Recombinant tagged full length human SF3B2 protein at 0.1 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 98 kDa
    Observed band size : 96 kDa (why is the actual band size different from the predicted?)
  • Anti-SF3B2 antibody (ab77154) at 1 µg/ml + HeLa nuclear lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 98 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)

References for Anti-SF3B2 antibody (ab77154)

ab77154 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77154.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"