Overview

  • Product nameAnti-SF3B3 antibody
    See all SF3B3 primary antibodies
  • Description
    Rabbit polyclonal to SF3B3
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1167 and 1217 of Human SF3B3 (NP_036558.3).

  • Positive control
    • Human breast carcinoma, colon carcinoma, non-small cell lung cancer, ovarian carcinoma, prostate carcinoma or stomach adenocarcinoma tissue. Mouse colon carcinoma CT26, hybridoma tumor, renal cell carcinoma or teratoma tissue.
  • General notesShelf Life: 1 year from date of receipt

Properties

Applications

Our Abpromise guarantee covers the use of ab124658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionSubunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • Sequence similaritiesBelongs to the RSE1 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0017 antibody
    • Pre mRNA splicing factor SF3b 130 kDa subunit antibody
    • Pre-mRNA-splicing factor SF3b 130 kDa subunit antibody
    • RSE1 antibody
    • SAP 130 antibody
    • SAP130 antibody
    • SF3b130 antibody
    • SF3B3 antibody
    • SF3B3_HUMAN antibody
    • Spliceosome associated protein 130 antibody
    • Spliceosome-associated protein 130 antibody
    • Splicing factor 3b subunit 3 130kD antibody
    • Splicing factor 3B subunit 3 antibody
    • STAF130 antibody
    see all

Anti-SF3B3 antibody images

  • ab124658, at 1/500 dilution, staining SF3B3 in Formalin-fixed Paraffin-embedded Human ovarian carcinoma tissue, by Immunohistochemistry. Detection by DAB staining.
  • ab124658, at 1/100 dilution, staining SF3B3 in Formalin-fixed Paraffin-embedded Mouse renal cell carcinoma tissue, by Immunohistochemistry. Detection by DAB staining.

References for Anti-SF3B3 antibody (ab124658)

ab124658 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"