Overview

  • Product nameAnti-SF3B3 antibody
    See all SF3B3 primary antibodies
  • Description
    Rabbit polyclonal to SF3B3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 1152-1201 (HPPLCGRDHL SFRSYYFPVK NVIDGDLCEQ FNSMEPNKQK NVSEELDRTP) of Human SF3B3 (NP_036558).

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 136 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionSubunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • Sequence similaritiesBelongs to the RSE1 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0017 antibody
    • Pre mRNA splicing factor SF3b 130 kDa subunit antibody
    • Pre-mRNA-splicing factor SF3b 130 kDa subunit antibody
    • RSE1 antibody
    • SAP 130 antibody
    • SAP130 antibody
    • SF3b130 antibody
    • SF3B3 antibody
    • SF3B3_HUMAN antibody
    • Spliceosome associated protein 130 antibody
    • Spliceosome-associated protein 130 antibody
    • Splicing factor 3b subunit 3 130kD antibody
    • Splicing factor 3B subunit 3 antibody
    • STAF130 antibody
    see all

Anti-SF3B3 antibody images

  • Anti-SF3B3 antibody (ab86992) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 136 kDa
    Observed band size : 136 kDa
    Additional bands at : 60 kDa,65 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SF3B3 antibody (ab86992)

ab86992 has not yet been referenced specifically in any publications.

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