Overview

  • Product name
  • Description
    Rabbit polyclonal to SF3B4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 374-424 of Human SF3B4 (NP_005841.1).

  • Positive control
    • HeLa, 293T, NIH 3T3 and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/1000. Predicted molecular weight: 44 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-SF3B4 antibody (ab157117) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 44 kDa


    Exposure time: 10 seconds
  • Immunoprecipitation analysis of whole cell lysate from 293T cells (1 mg for IP; 20% of IP loaded), labeling SF3B4 with ab157117 at 6 µg/mg lysate (lane 2). Immunoprecipitation of SF3B4 was also tested using an alternative Rabbit anti-SF3B4 (lane 1), or control IgG (lane 3). For blotting immunoprecipitated SF3B4, ab157117 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab157117 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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