Overview

  • Product name
  • Description
    Rabbit polyclonal to SFMBT1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 816-866 of Human SFMBT1 (NP_057413).

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab114863 was affinity purified using an epitope specific to SFMBT1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab114863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 98 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Tissue specificity
    Expressed in all cell lines and normal tissues tested, including the thymus.
  • Sequence similarities
    Contains 4 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434L243 antibody
    • MGC93349 antibody
    • OTTMUSP00000030378 antibody
    • OTTMUSP00000030379 antibody
    • OTTMUSP00000030381 antibody
    • Renal ubiquitous protein 1 antibody
    • RU1 antibody
    • Scm like with four mbt domains 1 antibody
    • Scm like with four MBT domains protein 1 antibody
    • Scm related gene containing four mbt domains antibody
    • Scm related gene product containing four mbt domains antibody
    • Scm-like with four MBT domains protein 1 antibody
    • SFMBT antibody
    • SFMBT 1 antibody
    • Sfmbt1 antibody
    • SMBT1_HUMAN antibody
    • Smr antibody
    see all

Images

  • All lanes : Anti-SFMBT1 antibody (ab114863) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 98 kDa


    Exposure time : 3 minutes
  • Detection of SFMBT1 by Western Blot of Immunprecipitate.
    ab114863 at 1µg/ml staining SFMBT1 in HeLa whole cell lysate immunoprecipitated using ab114863 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab114863 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell)
Gel Running Conditions
Reduced Denaturing (3-8% gel)
Loading amount
5 µg
Specification
HeLa cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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Verified customer

Submitted Dec 01 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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