Overview

  • Product nameAnti-SFPQ antibody
    See all SFPQ primary antibodies
  • Description
    Rabbit polyclonal to SFPQ
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: MYQKERETPP RFAQHGTFEY EYSQRWKSLD EMEKQQREQV EKNMKDAKDK, corresponding to internal sequence amino acids 469-518 of Human SFPQ

  • Positive control
    • Jurkat cell lysate (Western blot). Human skeletal muscle (IHC-P).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: Use at an assay dependent dilution. (ELISA titre using peptide based assay: 1/1562500)
    IHC-P: Use at a concentration of 4.0 - 8.0 µg/ml.
    WB: Use at a concentration of 5.0 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
    • Involvement in diseaseNote=A chromosomal aberration involving SFPQ may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;1)(p11.2;p34) with TFE3.
    • Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
    • Post-translational
      modifications
      The N-terminus is blocked.
      Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA.
      Arg-7, Arg-9, Arg-19 and Arg-25 are dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localizationNucleus matrix. Predominantly in nuclear matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • 100 kDa DNA pairing protein antibody
      • 100 kDa DNA-pairing protein antibody
      • 100 kDa subunit antibody
      • DNA binding p52/p100 complex 100 kDa subunit antibody
      • DNA-binding p52/p100 complex antibody
      • hPOMp100 antibody
      • Polypyrimidine tract binding protein associated splicing factor antibody
      • Polypyrimidine tract-binding protein-associated-splicing factor antibody
      • POMP100 antibody
      • PPP1R140 antibody
      • proline- and glutamine-rich antibody
      • Protein phosphatase 1 regulatory subunit 140 antibody
      • PSF antibody
      • PTB associated splicing factor antibody
      • PTB-associated-splicing factor antibody
      • Sfpq antibody
      • SFPQ_HUMAN antibody
      • Splicing factor antibody
      • Splicing factor proline and glutamine rich antibody
      • Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antibody
      • Splicing factor proline/glutamine rich antibody
      see all

    Anti-SFPQ antibody images

    • Anti-SFPQ antibody (ab56050) at 5 µg/ml + Jurkat cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 76 kDa
      Observed band size : 76 kDa
    • ab56050, at 8µg/ml, staining Human SFPQ in muscle by immunohistochemistry on paraffin embedded tissue. The arrows indicate positively stained skeletal muscle cells. Magnification 400X.
    • Immunohistochemistry of Human Muscle lysate tissue at an antibody concentration of 4-8µg/ml using anti-SFPQ antibody (ab56050)

    References for Anti-SFPQ antibody (ab56050)

    ab56050 has not yet been referenced specifically in any publications.

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