Overview

  • Product name
    Anti-SFPQ antibody [B92]
    See all SFPQ primary antibodies
  • Description
    Mouse monoclonal [B92] to SFPQ
  • Specificity
    By immunoblotting of mouse cells of immature myeloid origin (bone marrow and acute myeloid leukemia), the antibody identifies a 47 kDa protein, with an apparent M.W. of 49 kDa. In lymphoid cells, peripheral blood cells and other tissues, it recognizes a major 100 kDa antigen and a cleavage product of 68 kDa. In HeLa cells, bands of 120, 100, 80 and 68 kDa are stained by the antibody. It stains immature granulocyte nuclei in a speckled pattern characteristic of pre-mRNA splicing factors.
  • Tested applications
    Suitable for: ICC/IF, IP, RIA, ICC, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Tissue/ cell preparation (lysate of mouse bone marrow-derived stromal cell line.).

  • Positive control
    • Whole extract of cultured HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    B92
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20082308
IP Use at an assay dependent concentration. (see Lee et al reference, Carramolino et al reference).
RIA Use at an assay dependent concentration. (see Lee et al reference).
AP Use at an assay dependent concentration. (see Shav-Tal et al reference).
ICC Use at an assay dependent concentration. (see Lee et al reference).
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 47, 49, 100, 68, 120, 100, 80, 68 kDa (predicted molecular weight: 76 kDa). (See Lee et al reference, Shav-Tal et al reference).

Target

  • Function
    DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
  • Involvement in disease
    Note=A chromosomal aberration involving SFPQ may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;1)(p11.2;p34) with TFE3.
  • Sequence similarities
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    The N-terminus is blocked.
    Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA.
    Arg-7, Arg-9, Arg-19 and Arg-25 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Nucleus matrix. Predominantly in nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 100 kDa DNA pairing protein antibody
    • 100 kDa DNA-pairing protein antibody
    • 100 kDa subunit antibody
    • DNA binding p52/p100 complex 100 kDa subunit antibody
    • DNA-binding p52/p100 complex antibody
    • hPOMp100 antibody
    • Polypyrimidine tract binding protein associated splicing factor antibody
    • Polypyrimidine tract-binding protein-associated-splicing factor antibody
    • POMP100 antibody
    • PPP1R140 antibody
    • proline- and glutamine-rich antibody
    • Protein phosphatase 1 regulatory subunit 140 antibody
    • PSF antibody
    • PTB associated splicing factor antibody
    • PTB-associated-splicing factor antibody
    • Sfpq antibody
    • SFPQ_HUMAN antibody
    • Splicing factor antibody
    • Splicing factor proline and glutamine rich antibody
    • Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antibody
    • Splicing factor proline/glutamine rich antibody
    see all

References

This product has been referenced in:
  • Wang Z  et al. NEAT1 modulates herpes simplex virus-1 replication by regulating viral gene transcription. Cell Mol Life Sci 74:1117-1131 (2017). WB . Read more (PubMed: 27783096) »
  • Yasui DH  et al. Mice with an isoform-ablating Mecp2 exon 1 mutation recapitulate the neurologic deficits of Rett syndrome. Hum Mol Genet 23:2447-58 (2014). Read more (PubMed: 24352790) »

See all 11 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (lung cancer)
Total protein in input
5 µg
Immuno-precipitation step
Protein G
Specification
lung cancer
Username

Ms. Hannah Eischeid

Verified customer

Submitted Apr 25 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa and Mouse Embryonic fibroblast (MEF))
Loading amount
50 µg
Specification
HeLa and Mouse Embryonic fibroblast (MEF)
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 21 2007

Thank you for your enquiry. I have updated the datasheet with all the information I was able to find on this antibody, you will find references where the clone has been used (this is the same clone, not from Abcam therefore dilutions will not be the...

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Thank you for your enquiry. Unfortunately, we don't sell small aliquots of this product. At the moment we are out of the lot number you require. I offer you my sincerest apologies for any troubles you've had with the above mentioned lots. It's quite od...

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