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IGPYQSGRFYTKC, corresponding to amino acids 42 - 53 of Human SFRP1.
Our Abpromise guarantee covers the use of ab4193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Boil the sections for 20-30 minutes in water bath. We also recomend trying different antigen retrieval methods with different buffers if one does not work.
|WB||1/230. Predicted molecular weight: 37 kDa.|
Western blot using ab4193 at 1/230 (o/n 4°C) on lysates from cultured small airway epithelial cells.
Becky Mercer, University of Columbia
ab4193 staining human dermal hypertrophic scar tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval prior to blocking (with a commercial protein block) and incubating with the primary antibody (diluted 1/800) for 20 minutes. A HRP-conjugated goat anti-rabbit antibody was used as the secondary.
ab4193 staining SFRP1 by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in human breast cancer cells, injected subcutaneously into mice and grown until the tumour was a palpable size. Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in 10mM sodium citrate- EDTA (pH 6). The primary antibody was diluted 1/1000 and incubated with sample for 17 hours at 4°C in 10% Casein. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
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