Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab4193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil the sections for 20-30 minutes in water bath. We also recomend trying different antigen retrieval methods with different buffers if one does not work.

WB 1/230. Predicted molecular weight: 37 kDa.

Target

  • FunctionSoluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
  • Tissue specificityWidely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
  • Sequence similaritiesBelongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • DomainThe FZ domain is involved in binding with Wnt ligands.
  • Cellular localizationSecreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled related protein 1 antibody
    • FRP 1 antibody
    • FRP antibody
    • FRP-1 antibody
    • FRP1 antibody
    • FrzA antibody
    • SARP 2 antibody
    • SARP-2 antibody
    • SARP2 antibody
    • Secreted apoptosis related protein 2 antibody
    • Secreted apoptosis-related protein 2 antibody
    • Secreted frizzled related protein 1 antibody
    • Secreted frizzled related protein antibody
    • Secreted frizzled-related protein 1 antibody
    • SFRP 1 antibody
    • sFRP-1 antibody
    • SFRP1 antibody
    • SFRP1_HUMAN antibody
    see all

Anti-SFRP1 antibody images



  • Predicted band size : 37 kDa


    Western blot using ab4193 at 1/230 (o/n 4°C) on lysates from cultured small airway epithelial cells.

    Becky Mercer, University of Columbia

  • ab4193 staining human dermal hypertrophic scar tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval prior to blocking (with a commercial protein block) and incubating with the primary antibody (diluted 1/800) for 20 minutes.  A HRP-conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ICC/IF image of ab4193 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4193, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab4193 staining SFRP1 by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in human breast cancer cells, injected subcutaneously into mice and grown until the tumour was a palpable size. Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in 10mM sodium citrate- EDTA (pH 6). The primary antibody was diluted 1/1000 and incubated with sample for 17 hours at 4°C in 10% Casein. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.

    See Abreview

  • ab4193 detecting SFRP1 in Human Tear samples (purified protein) by sandwich ELISA. Protein was captured and Samples were blocked with 3% milk for 2 hours at 25°C and incubated with ab4193 (1/1000 in PBS ) for 1 hour at 37°C. An alkaline phosphatase Goat anti-rabbit IgG polyclonal (1/15000) was used as the secondary antibody.

    See Abreview

References for Anti-SFRP1 antibody (ab4193)

This product has been referenced in:
  • Zuckermann M  et al. Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling. Nat Commun 6:7391 (2015). IHC-P ; Mouse . Read more (PubMed: 26067104) »
  • Valcz G  et al. Myofibroblast-derived SFRP1 as potential inhibitor of colorectal carcinoma field effect. PLoS One 9:e106143 (2014). IHC-P ; Human . Read more (PubMed: 25405986) »

See all 11 Publications for this product

Product Wall

Your protocol appears to be correct. The only IHC data we have is from customers who used citrate heat-mediated retrieval and dilutions of 1/1000 or 1/800. The samples were hypertrophic scar and a subcutaneous implant of MDA-MB 436 breast cancer cells....

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The highest expression is in the heart and kidney. For an example of a strong stain of heart muscle tissue, please see the image on the datasheet for a different SFRP1 antibody, ab126613:

Click here (or use the following: http://www.abcam.co...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital ...

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Thank you very much for providing that information. It has been very helpful in understanding the results you have seen so far in your experiment.

I am sorry but Kate is currently away from the office but I will try my best to help you with th...

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Thank you for taking the time to reply with the information.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to r...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibody.

I would like to reassure you that our antibodies are tested and covered by our 6 month guarantee for ...

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Este es un mail de confirmación del nuevo pedido, consistente en el reemplazo completamente gratuito, del anticuerpo ab4193 con número de pedido XXX.

Os llegará un mail de confirmación del nuevo pedido, si necesit&...

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Gracias por contactarnos y alertarnos del problema sufrido con este anticuerpo.

Efectivamente los resultados obtenidos con este nuevo lote no se corresponden con los esperados, y le pido disculpas por las molestias que esto haya podido ocasio...

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Purified protein (Tears)
Specification Tears
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Type Sandwich (Detection)
Username

Abcam user community

Verified customer

Submitted Aug 24 2012

Thank you for your email.

I apologize for the delay as it took a while to get back to you. Unfortunately the publication team at Abcam is unable to trace back the antibodies catalogue number the author have used. They can only trace one abID w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"