Recombinant
RabMAb

Anti-SFRP1 antibody [EPR7003] (ab126613)

Overview

  • Product name
    Anti-SFRP1 antibody [EPR7003]
    See all SFRP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7003] to SFRP1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SFRP1. (Uniprot: Q8N474).

  • Positive control
    • Human heart, MDA-MB-435 and A375 lysates; Human heart muscle tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 25033833
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
    • Tissue specificity
      Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
    • Sequence similarities
      Belongs to the secreted frizzled-related protein (sFRP) family.
      Contains 1 FZ (frizzled) domain.
      Contains 1 NTR domain.
    • Domain
      The FZ domain is involved in binding with Wnt ligands.
    • Cellular localization
      Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • Frizzled related protein 1 antibody
      • FRP 1 antibody
      • FRP antibody
      • FRP-1 antibody
      • FRP1 antibody
      • FrzA antibody
      • SARP 2 antibody
      • SARP-2 antibody
      • SARP2 antibody
      • Secreted apoptosis related protein 2 antibody
      • Secreted apoptosis-related protein 2 antibody
      • Secreted frizzled related protein 1 antibody
      • Secreted frizzled related protein antibody
      • Secreted frizzled-related protein 1 antibody
      • SFRP 1 antibody
      • sFRP-1 antibody
      • SFRP1 antibody
      • SFRP1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : MDA-MB-435 lysate
      Lane 3 : A375 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 35 kDa
    • ab126613, at 1/50 dilution, staining SFRP1 in Formalin-fixed, Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Zhang R  et al. A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Nature 535:164-8 (2016). Read more (PubMed: 27383988) »
    • Zheng L  et al. Arsenic trioxide inhibits viability and induces apoptosis through reactivating the Wnt inhibitor secreted frizzled related protein-1 in prostate cancer cells. Onco Targets Ther 9:885-94 (2016). Read more (PubMed: 26966376) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse lung fibroblasts (MLg2908))
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Specification
    Mouse lung fibroblasts (MLg2908)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Arunima Seal

    Verified customer

    Submitted Sep 05 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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