Recombinant
RabMAb

Anti-SFRP1 antibody [EPR7003] (ab126613)

Overview

  • Product name
    Anti-SFRP1 antibody [EPR7003]
    See all SFRP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7003] to SFRP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SFRP1. (Uniprot: Q8N474).

  • Positive control
    • Human heart, MDA-MB-435 and A375 lysates; Human heart muscle tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
    • Tissue specificity
      Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
    • Sequence similarities
      Belongs to the secreted frizzled-related protein (sFRP) family.
      Contains 1 FZ (frizzled) domain.
      Contains 1 NTR domain.
    • Domain
      The FZ domain is involved in binding with Wnt ligands.
    • Cellular localization
      Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • Frizzled related protein 1 antibody
      • FRP 1 antibody
      • FRP antibody
      • FRP-1 antibody
      • FRP1 antibody
      • FrzA antibody
      • SARP 2 antibody
      • SARP-2 antibody
      • SARP2 antibody
      • Secreted apoptosis related protein 2 antibody
      • Secreted apoptosis-related protein 2 antibody
      • Secreted frizzled related protein 1 antibody
      • Secreted frizzled related protein antibody
      • Secreted frizzled-related protein 1 antibody
      • SFRP 1 antibody
      • sFRP-1 antibody
      • SFRP1 antibody
      • SFRP1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling SFRP1 with Purified ab126613 at 1:50 dilution (8 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution (purified) + MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysates at 15 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 35 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/5000 dilution (purified)

      Lane 1 : Human fetal kidney lysates
      Lane 2 : Human fetal heart lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 35 kDa
      Observed band size: 36 kDa (why is the actual band size different from the predicted?)



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution (unpurified)

      Lane 1 : Human heart lysate
      Lane 2 : MDA-MB-435 lysate
      Lane 3 : A375 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 35 kDa

    • Unpurified ab126613, at 1/50 dilution, staining SFRP1 in Formalin-fixed, Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Song XL  et al. miR-1301-3p promotes prostate cancer stem cell expansion by targeting SFRP1 and GSK3ß. Biomed Pharmacother 99:369-374 (2018). WB, RT-PCR . Read more (PubMed: 29358129) »
    • Takata N  et al. Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism. Nat Commun 8:1339 (2017). Read more (PubMed: 29109536) »

    See all 9 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse lung fibroblasts (MLg2908))
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Specification
    Mouse lung fibroblasts (MLg2908)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Arunima Seal

    Verified customer

    Submitted Sep 05 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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