The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa).Can be blocked with Human SFRP4 peptide (ab32783).
Use at an assay dependent concentration. PubMed: 25036934
FunctionSoluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
Tissue specificityExpressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).
Sequence similaritiesBelongs to the secreted frizzled-related protein (sFRP) family. Contains 1 FZ (frizzled) domain. Contains 1 NTR domain.
DomainThe FZ domain is involved in binding with Wnt ligands.
Cellular localizationSecreted. Cytoplasmic in ovarian tumor cells.
Mori K et al. Diabetic osteopenia by decreased ß-catenin signaling is partly induced by epigenetic derepression of sFRP-4 gene. PLoS One9:e102797 (2014).
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Ford CE et al. The Wnt gatekeeper SFRP4 modulates EMT, cell migration and downstream Wnt signalling in serous ovarian cancer cells. PLoS One8:e54362 (2013).
Read more (PubMed: 23326605) »