Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab32784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa).Can be blocked with Human SFRP4 peptide (ab32783).
ICC/IF Use at an assay dependent concentration. PubMed: 25036934

Target

  • FunctionSoluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
  • Tissue specificityExpressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).
  • Sequence similaritiesBelongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • DomainThe FZ domain is involved in binding with Wnt ligands.
  • Cellular localizationSecreted. Cytoplasmic in ovarian tumor cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled protein antibody
    • Frizzled protein human endometrium antibody
    • FRP 4 antibody
    • FRP4 antibody
    • FrpHE antibody
    • human endometrium antibody
    • Secreted frizzled-related protein 4 antibody
    • sFRP-4 antibody
    • Sfrp4 antibody
    • SFRP4_HUMAN antibody
    see all

Anti-SFRP4 antibody images

  • All lanes : Anti-SFRP4 antibody (ab32784) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-SFRP4 antibody (ab32784)

This product has been referenced in:
  • Mori K  et al. Diabetic osteopenia by decreased ß-catenin signaling is partly induced by epigenetic derepression of sFRP-4 gene. PLoS One 9:e102797 (2014). ICC/IF ; Mouse . Read more (PubMed: 25036934) »
  • Ford CE  et al. The Wnt gatekeeper SFRP4 modulates EMT, cell migration and downstream Wnt signalling in serous ovarian cancer cells. PLoS One 8:e54362 (2013). WB . Read more (PubMed: 23326605) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab32784.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"