Overview

  • Product name
  • Description
    Rabbit polyclonal to SFRS17A
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 475-525 of Human SFRS17A (NP_005079.2)

  • Positive control
    • HeLa, Jurkat, 293T cell lysates
  • General notes
    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99.91% Tris citrate/phosphate
    Note: pH: 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab114128 was affinity purified using an epitope specific to SFRS17A immobilized on a solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab114128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 81 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Splice factor regulating alternative splice site selection for certain mRNA precursors. Mediates regulation of pre-mRNA splicing in a PKA-dependent manner.
  • Tissue specificity
    Widely expressed. Found in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Expressed in activated B-cells and placenta. Expressed in all cell lines tested including Jurkat TAg, U937 and HEK293 cells.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain
    RI-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Cellular localization
    Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • 721P antibody
    • A-kinase anchor protein 17A antibody
    • AK17A_HUMAN antibody
    • AKAP-17A antibody
    • AKAP17A antibody
    • arginine/serine-rich 17A antibody
    • B lymphocyte antigen antibody
    • B lymphocyte surface antigen antibody
    • B-lymphocyte antigen antibody
    • CCDC133 antibody
    • PRKA17A antibody
    • Protein kinase A-anchoring protein 17A antibody
    • Protein XE7 antibody
    • Pseudoautosomal gene XE7 antibody
    • SFRS 17A antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 17A antibody
    • XE 7 antibody
    • XE7 antibody
    see all

Images

  • All lanes : Anti-SFRS17A antibody (ab114128) at 1/0.4 dilution

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 81 kDa


    Exposure time : 10 seconds
  • ab114128 at 6ug/mg of lysate detecting SFRS17A by immunoprecipitation (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control.
    Detection: chemiluminescence. Exposure time: 10 seconds.

References

ab114128 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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