The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at a concentration of 10 µg/ml.
Note: This product requires purification with Protein A prior to ICC/IF.
WB: 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight for isoform 3 is 52 kDa; and that for isoform 1 is 81 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSplice factor regulating alternative splice site selection for certain mRNA precursors. Mediates regulation of pre-mRNA splicing in a PKA-dependent manner.
Tissue specificityWidely expressed. Found in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Expressed in activated B-cells and placenta. Expressed in all cell lines tested including Jurkat TAg, U937 and HEK293 cells.