Overview

  • Product nameAnti-SFRS2B antibody
    See all SFRS2B primary antibodies
  • Description
    Rabbit polyclonal to SFRS2B
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (YRESRYGGSH YSSSGYSNSR YSRYHSSRSH SKSGSSTSSR SASTSKSSSA) of Human SFRS2B (NP_115285).

  • Positive control
    • 721_B cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionInvolved in pre-mRNA alternative splicing.
  • Tissue specificityStrongly expressed in pancreas, spleen and prostate. Weakly expressed in lung, liver and thymus.
  • Sequence similaritiesBelongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • arginine/serine-rich 2B antibody
    • Pre mRNA splicing factor SRP46 antibody
    • Pre-mRNA-splicing factor SRP46 antibody
    • Serine/arginine-rich splicing factor 8 antibody
    • SFRS2B antibody
    • Splicing factor antibody
    • Splicing factor arginine serine rich 46kD antibody
    • Splicing factor arginine serine rich 2B antibody
    • Splicing factor SRp46 antibody
    • SRP46 antibody
    • SRSF8 antibody
    • SRSF8_HUMAN antibody
    see all

Anti-SFRS2B antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling SFRS2B with ab83006 at 4.0-8.0µg/ml. Positive staining shown in cardiac cells. Magnification: 400X.
  • Anti-SFRS2B antibody (ab83006) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : 30 kDa,60 kDa,>90 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%

References for Anti-SFRS2B antibody (ab83006)

ab83006 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"