Overview

  • Product name
  • Description
    Rabbit polyclonal to SFRS3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to C terminal amino acids of Human SFRS3.

  • Positive control
    • MCF7 cells; HeLa and Jurkat cell extracts; Human Testis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+, Ca2+)
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.
ELISA 1/5000.

Target

  • Function
    May be involved in RNA processing in relation with cellular proliferation and/or maturation.
  • Sequence similarities
    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Extensively phosphorylated on serine residues in the RS domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • arginine/serine-rich 3 antibody
    • Pre mRNA splicing factor SRP20 antibody
    • Pre-mRNA-splicing factor SRP20 antibody
    • Serine/arginine-rich splicing factor 3 antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 20 kD antibody
    • Splicing factor arginine/serine rich 3 antibody
    • SRp20 antibody
    • SRSF3 antibody
    • SRSF3_HUMAN antibody
    • X16 antibody
    see all

Anti-SFRS3 antibody images



  • Performed under reducing conditions.

    Predicted band size : 19 kDa


    Exposure time : 5 seconds

    This image is courtesy of Suypriya Sen

    See Abreview

  • ab125124, at 5ug/ml, staining SFRS3 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-SFRS3 antibody (ab125124) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : Jurkat cell extract
    Lane 3 : Jurkat cell extract with synthesized peptide


    Predicted band size : 19 kDa
  • ab125124 at 1/100 dilution staining SRSF3 in MCF7 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

References for Anti-SFRS3 antibody (ab125124)

This product has been referenced in:
  • Sen S  et al. Deletion of splicing factor SRSF3 in hepatocytes predisposes to hepatocellular carcinoma in mice. Hepatology N/A:N/A (2014). Mouse . Read more (PubMed: 25132062) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Human Tissue sections (Liver)
Specification
Liver
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Suypriya Sen

Verified customer

Submitted Sep 05 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Mouse Tissue sections (liver)
Specification
liver
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Suypriya Sen

Verified customer

Submitted Aug 25 2014

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary Hepatocytes)
Loading amount
25 µg
Specification
Primary Hepatocytes
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Suypriya Sen

Verified customer

Submitted May 08 2013

Thank you for your enquiry.

I can confirm you are correct, these are the same antibody. We have sourced the same antibody from two different places and given them different product codes. The sources have tested them in different application...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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