Overview

  • Product nameAnti-SFRS3 antibody
    See all SFRS3 primary antibodies
  • Description
    Rabbit polyclonal to SFRS3
  • Tested applicationsSuitable for: IHC-Fr, ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal region of Human SFRS3

  • Positive control
    • WB: extracts of HeLa cells or Jurkat cells; ICC/IF: MCF7 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab73891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/500.
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionMay be involved in RNA processing in relation with cellular proliferation and/or maturation.
  • Sequence similaritiesBelongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Extensively phosphorylated on serine residues in the RS domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • arginine/serine-rich 3 antibody
    • Pre mRNA splicing factor SRP20 antibody
    • Pre-mRNA-splicing factor SRP20 antibody
    • Serine/arginine-rich splicing factor 3 antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 20 kD antibody
    • Splicing factor arginine/serine rich 3 antibody
    • SRp20 antibody
    • SRSF3 antibody
    • SRSF3_HUMAN antibody
    • X16 antibody
    see all

Anti-SFRS3 antibody images

  • ab73891 staining SFRS3 in mouse E9.5 embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA + 10% goat serum + 0.1% Triton X-100 in PBS for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 10% goat serum + 0.1% Triton) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-SFRS3 antibody (ab73891) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 19 kDa
    Observed band size : 19 kDa
  • Left panel: immunofluorescence analysis of MCF7 cells, using 1/500-1/1000 ab73891; Right panel: same, after pre-incubation with the immunizing peptide.

References for Anti-SFRS3 antibody (ab73891)

This product has been referenced in:
  • Wang Q  et al. Homer1 alternative splicing is regulated by gonadotropin-releasing hormone and modulates gonadotropin gene expression. Mol Cell Biol 34:1747-56 (2014). Read more (PubMed: 24591653) »
  • Sen S  et al. Splicing factor SRSF3 is crucial for hepatocyte differentiation and metabolic function. Nat Commun 4:1336 (2013). WB ; Mouse . Read more (PubMed: 23299886) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 100 µg
Treatment siRNA against SRSF3
Specification HeLa
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Nov 21 2016

Application Immunohistochemistry (Frozen sections)
Blocking step PBS, 1% BSA, 10% goat serum, 0.1% TX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Tissue sections (E9.5 embryo)
Specification E9.5 embryo
Permeabilization Yes - 0.5* Triton X-100
Fixative Formaldehyde
Username

Abcam user community

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Submitted Jan 30 2014

Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 5 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Suypriya Sen

Verified customer

Submitted Apr 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (HepG2 , HEK293)
Specification HepG2 , HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Suypriya Sen

Verified customer

Submitted Apr 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Fibroblast)
Loading amount 20 µg
Specification Human Fibroblast
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Thank you for your enquiry.

I can confirm you are correct, these are the same antibody. We have sourced the same antibody from two different places and given them different product codes. The sources have tested them in different application...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"