Overview

  • Product name
  • Description
    Rabbit polyclonal to SFRS3
  • Tested applications
    Suitable for: IHC-Fr, ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal region of Human SFRS3

  • Positive control
    • WB: extracts of HeLa cells or Jurkat cells; ICC/IF: MCF7 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab73891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/500.
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    May be involved in RNA processing in relation with cellular proliferation and/or maturation.
  • Sequence similarities
    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Extensively phosphorylated on serine residues in the RS domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • arginine/serine-rich 3 antibody
    • Pre mRNA splicing factor SRP20 antibody
    • Pre-mRNA-splicing factor SRP20 antibody
    • Serine/arginine-rich splicing factor 3 antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 20 kD antibody
    • Splicing factor arginine/serine rich 3 antibody
    • SRp20 antibody
    • SRSF3 antibody
    • SRSF3_HUMAN antibody
    • X16 antibody
    see all

Images

  • ab73891 staining SFRS3 in mouse E9.5 embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA + 10% goat serum + 0.1% Triton X-100 in PBS for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 10% goat serum + 0.1% Triton) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-SFRS3 antibody (ab73891) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 19 kDa
    Observed band size : 19 kDa
  • Left panel: immunofluorescence analysis of MCF7 cells, using 1/500-1/1000 ab73891; Right panel: same, after pre-incubation with the immunizing peptide.

References

This product has been referenced in:
  • Jiang P  et al. Fyn/heterogeneous nuclear ribonucleoprotein E1 signaling regulates pancreatic cancer metastasis by affecting the alternative splicing of integrin ß1. Int J Oncol 51:169-183 (2017). WB . Read more (PubMed: 28560430) »
  • Zheng X  et al. TRA2ß controls Mypt1 exon 24 splicing in the developmental maturation of mouse mesenteric artery smooth muscle. Am J Physiol Cell Physiol 308:C289-96 (2015). Read more (PubMed: 25428883) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
100 µg
Treatment
siRNA against SRSF3
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 21 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
PBS, 1% BSA, 10% goat serum, 0.1% TX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Tissue sections (E9.5 embryo)
Specification
E9.5 embryo
Permeabilization
Yes - 0.5* Triton X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 30 2014

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
5 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Suypriya Sen

Verified customer

Submitted Apr 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (HepG2 , HEK293)
Specification
HepG2 , HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Suypriya Sen

Verified customer

Submitted Apr 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Fibroblast)
Loading amount
20 µg
Specification
Human Fibroblast
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Thank you for your enquiry.

I can confirm you are correct, these are the same antibody. We have sourced the same antibody from two different places and given them different product codes. The sources have tested them in different application...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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