Anti-SFRS5 antibody (ab67175)


  • Product nameAnti-SFRS5 antibody
    See all SFRS5 primary antibodies
  • Description
    Mouse polyclonal to SFRS5
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length SFRS5 protein (Human)

  • Positive control
    • Human liver tissue lysate. Human ovary tissue sections. Hela cells. SFRS5 transfected 293T cell lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab67175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 31 kDa).
IHC-P Use a concentration of 1.5 µg/ml.
ICC/IF Use at an assay dependent dilution. NOTE: Product requires purification with Protein A prior to use in ICC/IF and IHC-P.


  • RelevanceSFRS5 plays a role in constitutive splicing and can modulate the selection of alternative splice sites. It is found in a pre-mRNA splicing complex with SFRS4, SNRP70, SNRPA1, SRRM1 and SRRM2. There are four isoforms.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Delayed early protein HRS antibody
    • HRS antibody
    • Pre mRNA splicing factor SRP40 antibody
    • Serine and arginine rich splicing factor 5 antibody
    • SFRS 5 antibody
    • Splicing factor arginine/serine rich 5 antibody
    • SRP 40 antibody
    • SRP40 antibody
    see all

Anti-SFRS5 antibody images

  • Anti-SFRS5 antibody (ab67175) at 1/500 dilution + human liver tissue lysate at 25 µg/ml

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SFRS5 antibody (ab67175) at 1/500 dilution

    Lane 1 : SFRS5 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
  • ab67175, at 1.5µg/ml, staining SFRS5 in human ovary by immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • ICC/IF image of ab67175 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67175, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-SFRS5 antibody (ab67175)

ab67175 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (lymphocytes)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 14 µg
Specification lymphocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 25 2016

Thank you for your email. The antibody is tested in Hela cells, liver tissue lysates and SFRS5 overexpressing 293T lysates. I haven’t included hela in my last email because we do not have images for comparison. I suggest trying the recommended steps. I...

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Thank you for your enquiry regarding ab67175 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. The protocol looks fine to me however I would like to make...

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