Anti-SFRS7/SRSF7 antibody (ab72616)
Key features and details
- Mouse polyclonal to SFRS7/SRSF7
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SFRS7/SRSF7 antibody
See all SFRS7/SRSF7 primary antibodies -
Description
Mouse polyclonal to SFRS7/SRSF7 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human SFRS7/SRSF7. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_001026854, 1 a.a. ~ 238 a.a)
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Positive control
- SFRS7/SRSF7 transfected lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro. Represses the splicing of MAPT/Tau exon 10. -
Tissue specificity
Brain, liver, kidney and lung. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 1 CCHC-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsExtensively phosphorylated on serine residues in the RS domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6432 Human
- Omim: 600572 Human
- SwissProt: Q16629 Human
- Unigene: 309090 Human
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Alternative names
- 9G8 antibody
- AAG3 antibody
- arginine/serine-rich 7 antibody
see all
Images
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Immunocytochemistry of HeLa cells staining SFRS7/SRSF7 using ab72616 at 10 μg/ml.
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All lanes : Anti-SFRS7/SRSF7 antibody (ab72616) at 1/500 dilution
Lane 1 : SFRS7/SRSF7 transfected 293T cell line
Lane 2 : Non transfected 293T cell line
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab72616 has not yet been referenced specifically in any publications.