Overview

  • Product nameAnti-SFXN1 antibody
    See all SFXN1 primary antibodies
  • Description
    Rabbit polyclonal to SFXN1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (SGELPPNINI KEPRWDQSTF IGRANHFFTV TDPRNILLTN EQLESARKIV) of human SFXN1 (NP_073591).

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-SFXN1 antibody images

  • Anti-SFXN1 antibody (ab86552) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : ~32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : ~57 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SFXN1 antibody (ab86552)

ab86552 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 50 µg
Specification HepG2
Gel Running Conditions Reduced Denaturing (12%)
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Apr 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"