Overview

  • Product nameAnti-SGK196 antibody
    See all SGK196 primary antibodies
  • Description
    Mouse monoclonal to SGK196
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: GDFVAPEQLW PYGEDVPFHD DLMPSYDEKI DIWKIPDISS FLLGHIEGSD MVRFHLFDIH KACKSQTPSE RPTAQDVLET YQKVLDTLRD AMMSQAREML , corresponding to amino acids 251-351 of Human FLJ23356

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent dilution.

Target

  • FunctionProtein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif.
  • Involvement in diseaseMuscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies A12
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. STKL subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ23356 antibody
    • MDDGA12 antibody
    • POMK antibody
    • Probable inactive protein kinase-like protein SgK196 antibody
    • Protein kinase like protein SgK196 antibody
    • Protein kinase-like protein SgK196 antibody
    • Protein O-mannose kinase antibody
    • SG196_HUMAN antibody
    • SGK196 antibody
    • Sugen kinase 196 antibody
    see all

Anti-SGK196 antibody images



  • Predicted band size : 40 kDa
  • ab57908 at 10µg/ml staining FLJ23356 in human Hela cells by ICC/IF (Immunocytochemistry / Immunofuorescence).

  • Detection limit for recombinant tagged (proprietary) SGK196 is approximately 0.1ng/ml as a capture antibody.

References for Anti-SGK196 antibody (ab57908)

This product has been referenced in:
  • Di Costanzo S  et al. POMK mutations disrupt muscle development leading to a spectrum of neuromuscular presentations. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 24925318) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"