Overview

  • Product nameAnti-SGK2 antibody
    See all SGK2 primary antibodies
  • Description
    Mouse monoclonal to SGK2
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: SPINWDDLYH KRLTPPFNPN VTGPADLKHF DPEFTQEAVS KSIGCTPDTV ASSSGASSAF LGFSYAPEDD DILDC, corresponding to amino acids 293-376 of human SGK2 (AAH65511) with a proprietary tag.

  • Positive control
    • WB: HepG2 and Raw 264.7 cell lysates ICC/IF: HeLa cell

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG3
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionInvolved in the activation of potassium channels.
  • Tissue specificityHighly expressed in liver, kidney and pancreas, and at lower levels in brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DJ138B7.2 antibody
    • H SGK2 antibody
    • HSGK2 antibody
    • Serine/threonine protein kinase SGK2 antibody
    • Serine/threonine-protein kinase Sgk2 antibody
    • Serum/glucocorticoid regulated kinase 2 antibody
    • Serum/glucocorticoid-regulated kinase 2 antibody
    • SGK2 antibody
    • SGK2_HUMAN antibody
    see all

Anti-SGK2 antibody images

  • Anti-SGK2 antibody (ab89144) at 5 µg/ml + tagged, recombinant human SGK2 protein (the immunogen) at 0.2 µg

    Predicted band size : 48 kDa
  • Anti-SGK2 antibody (ab89144) at 5 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 78 kDa. We are unsure as to the identity of these extra bands.
  • Anti-SGK2 antibody (ab89144) at 5 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • ab89144, at 10 µg/ml, staining SGK2 in HeLa cell by Immunofluorescence.
  • Detection limit for recombinant tagged SGK2 is approximately 0.1 ng/ml as a capture antibody.

References for Anti-SGK2 antibody (ab89144)

ab89144 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"