The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionActively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
Tissue specificityExpressed mainly in intestine and kidney.
Involvement in diseaseCongenital glucose/galactose malabsorption
Sequence similaritiesBelongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
Post-translational modificationsN-glycosylation is not necessary for the cotransporter function.