• Product nameAnti-SGLT1 antibody
    See all SGLT1 primary antibodies
  • Description
    Rabbit polyclonal to SGLT1
  • Tested applicationsSuitable for: IHC-P, WB, Dot Blot, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 603-623 of Human SGLT1



Our Abpromise guarantee covers the use of ab14685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 55-62 kDa.
Dot Blot 1/4000.
ELISA 1/4000.


  • FunctionActively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • Tissue specificityExpressed mainly in intestine and kidney.
  • Involvement in diseaseCongenital glucose/galactose malabsorption
  • Sequence similaritiesBelongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • Post-translational
    N-glycosylation is not necessary for the cotransporter function.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • D22S675 antibody
    • High affinity sodium glucose cotransporter 1 antibody
    • High affinity sodium glucose cotransporter antibody
    • High affinity sodium-glucose cotransporter antibody
    • Human Na+/glucose cotransporter 1 antibody
    • Na(+)/glucose cotransporter 1 antibody
    • Na+/glucose cotransporter 1 antibody
    • NAGT antibody
    • SC5A1_HUMAN antibody
    • SGLT 1 antibody
    • SGLT1 antibody
    • SLC5A1 antibody
    • Sodium glucose cotransporter 1 antibody
    • Sodium/glucose cotransporter 1 antibody
    • Solute carrier family 5 (sodium/glucose cotransporter) member 1 antibody
    • Solute carrier family 5 member 1 antibody
    see all

Anti-SGLT1 antibody images

  • ab14685 (2µg/ml) staining SGLT1 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the apical epithelium cells of the villi.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-SGLT1 antibody (ab14685)

This product has been referenced in:
  • Kashiwagi Y  et al. Expression of SGLT1 in Human Hearts and Impairment of Cardiac Glucose Uptake by Phlorizin during Ischemia-Reperfusion Injury in Mice. PLoS One 10:e0130605 (2015). Mouse . Read more (PubMed: 26121582) »
  • Pulskens WP  et al. Nlrp3 prevents early renal interstitial edema and vascular permeability in unilateral ureteral obstruction. PLoS One 9:e85775 (2014). IHC-P ; Mouse . Read more (PubMed: 24454932) »

See all 10 Publications for this product

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Thank you for your inquiry. I can confirm that rat SGLT1 has a predicted  molecular weight of ca. 73kDa. Molecular weights on gel are always only a rough estimate since the gel and buffer conditions can influence the resulting molecular weight of a pro...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Bladder cancer cells)
Loading amount 60 µg
Specification Human Bladder cancer cells
Treatment Betulinic acid, curcumin, Gefitinib for 48 hrs
Gel Running Conditions Reduced Denaturing (7%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Aug 16 2010

Thank you for your patience. For ab14685 the immunogen used was a synthetic peptide, corresponding to amino acids 603-623 of Human SGLT1. For ab14686 the immunogen used was a synthetic peptide, corresponding to amino acids 402-422 of Human SGLT1. Pl...

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The synthetic peptide sequence was: "RRA YDL FCG LEQ HGA PKM TEE C". The peptide was cyclize by disulfide bond between Cys 8 and Cys 22.